Time-lapse and immunostaining images of a U-shaped-hatching blastocyst that hatched completely, in which an initial “8”-shaped hatching occurred near the ICM.
- Yohei Onodera (1498519)
- Kazumasa Takahashi (3910708)
- Mayumi Goto (3910705)
- Mibuki Anzai (3910711)
- Natsuki Ono (3910717)
- Hiromitsu Shirasawa (3910720)
- Wataru Sato (149080)
- Hiroshi Miura (3910723)
- Naoki Sato (78889)
- Akira Sato (210501)
- Yukiyo Kumazawa (3910714)
- Yukihiro Terada (149090)
Publication date
April 2017
DOIAbstract
<p>(<b>A</b>) At 90 h 45 min after insemination. Near the ICM (surrounded by arrowheads), an “8”-shaped hatch appears (arrow). (<b>B</b>) At 117 h 57 min after insemination. U-shaped hatching appears (arrow) in this embryo. (<b>C</b>) At 119 h 47 min after insemination. The embryo has hatched completely. (<b>D</b>) Immunostaining at 120 h post-insemination. The result confirmed that the ICM, stained by anti-Oct3/4 (red), is scattered. Scale bar, 20 μm.</p
Add to ORKG